Genetic manipulations enable female embryo to develop as opposite sex, study shows
Researchers have created male mice with no trace of a Y chromosome, supposedly the defining hallmark of being male.
Reproductive biologist Monika Ward of the University of Hawaii in Honolulu and colleagues started with mice that have only one X chromosome (and no second sex chromosome). Normally those animals would develop as females. But when the researchers manipulated genes found on the X and another chromosome, the mice became males that could produce immature sperm. Those engineered males fathered offspring with reproductive assistance from the researchers, who injected the immature sperm into eggs, Ward and colleagues report in the Jan. 29 Science.
The experiments demonstrate that there are multiple ways to make males, says Richard Behringer, a developmental geneticist at MD Anderson Cancer Center in Houston. “They’ve done it without any Y chromosome gene information,” he