How to make a fish face, and other photo contest winners

Winning imaging technique allows geneticists to study development at cellular level

fish face

FISH FACE Geneticist Oscar Ruiz snapped this photo of a 4-day-old zebrafish embryo. The image won first place in the 2016 Nikon Small World contest.

O. Ruiz/Univ. of Texas MD Anderson Cancer Center

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This forlorn-looking face of a 4-day-old zebrafish embryo represents “a whole new avenue of research” for geneticist Oscar Ruiz, who studies how faces and facial abnormalities develop at the cellular level.  

The research is possible thanks to a new method, developed by Ruiz and colleagues at the University of Texas MD Anderson Cancer Center in Houston, for mounting embryos in a gel that allows for clear, head-on pictures. A technique called confocal microscopy captures images like the one above, the first-place winner of this year’s Nikon Small World photography contest.

The embryo was euthanized before having its picture taken. But Ruiz is experimenting with taking time-lapse images of live, anesthetized zebrafish embryos. The camera snaps an image every five minutes for up to 48 hours, meaning that “we’re able to watch the development happening,” Ruiz says.

So far, the team has looked at embryos ranging from 1 to 6 days old. The researchers are compiling the images into an atlas documenting the developmental stages of the zebrafish face. They plan to use CRISPR/Cas9, a powerful gene-editing tool, to alter genes involved in facial abnormalities in the fish and then watch what changes, if any, occur. The research could one day be used to help scientists understand how a cleft lip or cleft palate develops in humans and possibly help treat it, Ruiz says.

In the image above, shown at 10x magnification, basal cells (green) in the bottom layer of skin give rise to more developed surface skin cells (red). Cell nuclei appear in blue.

“Everyone’s first impression is that those two holes in the center are its developing eyes,” Ruiz says. But they’re not. Those deceptive hollows are nascent olfactory tissue, used to smell. The eyes are actually the big bulges on either side of the face.

Although this was the first time Ruiz entered the microscopy photography contest, he is an amateur photographer. Landscape or travel photos — not fish photos — are his subject matter of choice.

Here are more finalists and honorable mentions from the competition:

ROCK SOLID This image of a polished slab of Teepee Canyon agate, a 273-million-year-old marine sedimentary rock found in the Black Hills of western South Dakota, snagged second place in the competition. It’s shown at 90x magnification. Douglas L. Moore/Univ. of Wisconsin-Stevens Point Museum of Natural History
BRAIN GAME These nerve cells, or neurons (stained green), were grown in the lab from human skin cells. Schwann cells (stained red), another type of brain cell, from rodents and cell bodies (stained blue) are also depicted, offering a glimpse into the complex network of the brain. This image, shown at 20x magnification, placed third. Rebecca Nutbrown/Univ. of Oxford
PLAYING FOOTSIE The complex front foot of a male diving beetle (Acilius) is covered in suction cups of varied sizes (shown at 100x magnification), which help the aquatic insect attach to a female’s back when mating. (5th place) Igor Siwanowicz/HHMI Janelia Research Campus
LIGHT BRIGHT The iridescent scales of a butterfly wing (seen at 10x magnification) contain tiny crystalline structures that reflect light of different wavelengths. (Honorable mention) Evan Darling/Memorial Sloan Kettering Cancer Center
BITTY BUBBLES Melting crystals of ascorbic acid, also known as Vitamin C, created these air bubbles (shown at 50x magnification), which were photographed using polarized light. (6th place) Marek Miś
IN HIGH GEAR A close-up view of the hind legs of a planthopper nymph (at 250x magnification) shows tiny bumps on each leg that interlock like the teeth of a gear, helping the flightless insect make precise, lightning-quick jumps. (Honorable mention) Igor Siwanowicz/HHMI Janelia Research Campus
Emily DeMarco

Emily DeMarco is the deputy news editor. She has a bachelor's degree in English from Furman University and a master of environmental science and management from the University of California, Santa Barbara.

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