Laser light made inside cells
Microscopic implants trap, amplify light, letting researchers track cellular activity

LASER TAGS Seven of the cells in this micrograph contain lasers in the form of plastic beads (green). The cells are enclosed in red; the nuclei are blue.
Matjaž Humar and Seok-Hyun Yun
Biologists often use lasers to probe cells. Now, for the first time, cells have returned fire.
Harvard University researchers have created intracellular lasers by implanting microscopic beads and oil droplets into animal cells. When energized by an outside laser pulse, an implant traps and amplifies light and then emits a laser pulse of its own. “It’s a wonderful way of coupling optics to cells to learn about biological processes,” says chemist Richard Zare of Stanford University. The microscopic lasers, reported July 27 in Nature Photonics, could allow scientists to track the motion of thousands of individual cells.
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The new technique is far from the first to coax cells to generate their own light. Biologists routinely scrutinize cells under the microscope with the help of fluorescent dyes and proteins that glow when energized by an external laser pulse (SN Online: 10/8/14). But those cells emit light with a wide span of wavelengths, says physicist Seok-Hyun Yun. That can make it difficult to separate the cells’ glow from background illumination. Yun and colleague Matjaž Humar set out to implant cells with lasers, which emit light only at specific wavelengths.

The component wavelengths of the laser light depend on the size of the spheres, Yun says. Humar and Yun showed that they could measure cells’ internal pressure by charting changes in laser light that occur when cellular fluid compresses the oil droplets. The rigid beads, which don’t change in size, could be used to tag individual cells. By using beads that differ in diameter by as little as 2 nanometers, Yun says that scientists can distinguish between thousands of individual cells by their laser signature.